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Abstracts tumor DNA was obtained by QIAGEN FFPE kit. 12,13 and 61 codon in KRAS and 600 codon in BRAF were determined by routine PCR and pyrosequencing. Meanwhile, isolated DNA from tumor and matched normal tissues were detected by five microsatellites sites named BAT25,BAT26,D2S123,D5S346 and D17S250 by FM CE. Results.